New linear plots for the separate estimation of Michaelis-Menten parameters
نویسندگان
چکیده
منابع مشابه
The 'double' Michaelis-Menten equation: estimation of parameters.
When the concentration dependence of an enzymic reaction or a transport process can be described by the sum of two Michaelis-Menten terms, reliable data that relate reaction rate and substrate concentration can be obtained even when as much as 70 per cent of substrate was consumed during the assay. Each data pair consists of the average reaction rate during an assay and the concentration where ...
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The Michaelis-Menten equation, a rectangular hyperbola with two ~arameters ~o b~ specified, . occurs prominently 1n enzyme k,netlcs." Often, ~n consulting situations we need to (i) obtain estlmates standard errors, and confidence intervals for the unknown parameters, which define this nonlinear model for one set of experimental conditions and (ii) compare the parameter estimates under different...
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where K and V are some unknowns. For example, in study of chemical reactions, equation (1.1) is known as the Michaelis–Menten equation which describes the theoretical interrelation between the speed of reaction v(s) and the amount of reagent s. In this case the parameter V has the meaning of a maximally possible speed of reaction and the parameter K is the value of s for which the speed of reac...
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We study chemical reactions with complex mechanisms under two assumptions: (i) intermediates are present in small amounts (this is the quasi-steady-state hypothesis or QSS) and (ii) they are in equilibrium relations with substrates (this is the quasiequilibrium hypothesis or QE). Under these assumptions, we prove the generalized mass action law together with the basic relations between kinetic ...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1974
ISSN: 0014-5793
DOI: 10.1016/0014-5793(74)80734-9